AustralieFrV1, Main, Exploration, bibRecord, 006C00

Development, mapping and transferability of Fragaria EST-SSRs within the Rosodae supertribe

Identifieur interne : 006C00 ( Main/Exploration ); précédent : 006B99; suivant : 006C01

Development, mapping and transferability of Fragaria EST-SSRs within the Rosodae supertribe

Auteurs : M. Rousseau-Gueutin [France, Australie] ; L. Richard [France] ; L. Le Dantec [France] ; H. Caron [France] ; B. Denoyes-Rothan [France]

Source :

Plant breeding [ 0179-9541 ] ; 2011.

RBID : Pascal:11-0197131

Descripteurs français

Pascal (Inist)
- Cartographie, Marqueur EST, DNA microsatellite, Gène, Polymorphisme, Amélioration génétique, Fragaria, Fraise (fruit).
Wicri :
- topic : Cartographie.

English descriptors

KwdEn :
- Cartography, Expressed sequence tag, Fragaria, Gene, Genetic improvement, Microsatellite DNA, Polymorphism, Strawberry.

Abstract

The Rosodae supertribe is composed of economically important species (strawberry. rose and raspberry). In order to extend the repertoire of markers for optimizing genetic mapping and synteny within this supertribe. we developed 20 functional markers from two Fragaria cDNA libraries enriched in AG/AC microsatellite motifs. We tested their transferability within the Rosodae supertribe, including two reference diploid and octoploid strawberry segregating populations. Out of these 20 functional markers, 70% revealed polymorphism in both diploid and octoploid Fragaria segregating populations and were mapped on the diploid genetic map to provide useful information for further marker-assisted selection. These 20 EST-SSRs were also tested in 17 wild Fragaria species and displayed a high cross-species transferability (mean of 95%), providing new markers for further diversity studies within the genus. These markers were also transferable to the phylogenetically close Potenlilla genus, whereas their transferability was lower for the polyploid Duchesnea indica species and for the more distant Rosa genus. Their use for genetic mapping within the Rosodae will further permit a comparison of genetic information among the different genera.

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">The Rosodae supertribe is composed of economically important species (strawberry. rose and raspberry). In order to extend the repertoire of markers for optimizing genetic mapping and synteny within this supertribe. we developed 20 functional markers from two Fragaria cDNA libraries enriched in AG/AC microsatellite motifs. We tested their transferability within the Rosodae supertribe, including two reference diploid and octoploid strawberry segregating populations. Out of these 20 functional markers, 70% revealed polymorphism in both diploid and octoploid Fragaria segregating populations and were mapped on the diploid genetic map to provide useful information for further marker-assisted selection. These 20 EST-SSRs were also tested in 17 wild Fragaria species and displayed a high cross-species transferability (mean of 95%), providing new markers for further diversity studies within the genus. These markers were also transferable to the phylogenetically close Potenlilla genus, whereas their transferability was lower for the polyploid Duchesnea indica species and for the more distant Rosa genus. Their use for genetic mapping within the Rosodae will further permit a comparison of genetic information among the different genera.</div>
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Development, mapping and transferability of Fragaria EST-SSRs within the Rosodae supertribe

Development, mapping and transferability of Fragaria EST-SSRs within the Rosodae supertribe

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri